Journal: Acta Crystallographica Section D: Structural Biology
Article Title: Towards patient-relevant structures: reviewing body-temperature biological macromolecules and their ligands for pharmaceutical applications
doi: 10.1107/S2059798325010617
Figure Lengend Snippet: Line graph of the diffraction data-collection temperature (orange) of PDB structures that were determined at 310 K ( i.e. 37°C) and greater, together with the respective crystallization temperature (blue). Data from relevant crystallization studies are also included. Metadata details are available in Supplementary Tables S1 and S2.
Article Snippet: During the collection of diffraction data the temperature has so far been controlled via the CryoStream apparatus from Oxford Cryosystems ( https://oxcryo.com/products/cryostream/ ), which has a built-in heater device that achieves a range of crystal sample temperatures from 100 to 350 K. This device is very widely available, including at home X-ray laboratories, synchrotron beamlines and neutron macromolecular crystallography instruments.
Techniques: Crystallization Assay